anti-Caspase-7 Antibody (Clone B4-G2)

Caspase-7

CASP7,CASP-7,ICE-LAP3,CMH-1,MCH3, EC 3.4.22.60

The caspase family of cysteine proteases plays a key role in apoptosis. Caspase-7, also known as Mch3α, ICE/LAP3 and CMH1, is a 34 kDa protein that is activated during Fas- and TNF-induced apoptosis. Caspase-7 has been identified as a major contributor to the execution phase of cellular apoptosis. It cleaves essential cellular machinery following its own cleavage from an inactive proenzyme to an active heterodimeric form. Caspase-7 is processed by caspase-3, -6, -8, -9, and –10 as well as by Granzyme B.

Mouse

Monoclonal

Mouse IgG1

B4-G2

Human, mouse, rat

Synthetic peptide from human caspase-7.

0.5 mg/ml

Liquid

100 µg (0.5 µg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol

Affinity purified

Western blot, Immunoprecipitation

Western blot analysis (0.5-2 µg/ml) and immunoprecipitation (4-10 µg/ml). However the optimal conditions should be determined individually. Detects both pro-caspase-7 (34 kDa) and the large subunit of cleaved caspase-7 (19 kDa) in apoptosis cells on SDS-PAGE immunoblots.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps