anti-Caspase-1 Antibody

Caspase-1

CASP1, IL1BCE, P45, Interleukin-1 beta convertase, IL-1BC, IL1BC, p45, IL1B-convertase, CASP-1, ICE

Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-1 (ICE, interleukin-1β-converting enzyme) is the mammalian caspase responsible for the proteolytic conversion of the proforms of interleukin-1β and IL-18 into active cytokines. Like other caspases, Caspase-1 itself is synthesized as a pro-enzyme that is cleaved during activation into a large (20 kDa) and small (10 kDa) subunits. The cellular role of caspase-1 appears limited to cytokine activation as they do not have obvious roles in apoptosis.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 132 of human caspase-1

0.5 mg/ml

Liquid

30 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunofluorescence

Western blot analysis (0.5-4 µg/ml), immunoprecipitation (4-8 µg/ml) and immunofluorescence (10-20 µg/ml). The immunoaffinity purified antibody recognizes 45 kDa procaspase-1 and the 20 kDa cleaved product.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps