anti-Caspase-4 Antibody

Caspase-4

CASP-4, ICH-2 protease, TX protease, ICH2

Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as other autoimmune diseases. Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-4 is also known as Ich-2, TX, and ICErel-II and can proteolytically processed by granzyme B. Over expression of caspase-4 induces apoptosis in Rat-1 and HeLa cells, which can be inhibited by CrmA and Bcl-2.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 100 of human caspase-4

0.2 mg/ml

Liquid

30 µg (0.2 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot

Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The affinity purified antibody detects the 43 kDa proform of caspase-4.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps