anti-Bad Antibody

Bad

BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like 8 protein

The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bad) apoptosis. Similar to other family member, Bad also exhibits sequence homology to Bcl-2. Bad can selectively dimerize with Bcl-xL and Bcl-2, but not with Bax, Bcl-xs, Mcl-1, or itself. In mammalian cells, Bad binds more strongly to Bcl-xL than Bcl-2. This may explain why Bad reverse the death repressor activity of Bcl-xL, but not that of Bcl-2

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat, monkey

Synthetic peptide surrounding amino acid 115 of mouse Bad

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-Bad polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blot analysis (1-2 µg/ml), immunoprecipitation (4-8 µg/ml), and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the 22 kDa Bad protein and it does not cross-react with related family members.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps