anti-p53 Antibody (Clone C6A5)

p53

TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53

p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.

Mouse

Monoclonal

Mouse IgG1

C6A5

Human, mouse, rat

Synthetic peptide corresponding to residue surrounding Ser20 of human p53

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry

Western blotting (0.5-4 µg/ml), immunoprecipitation (4-8 µg/ml), Immunohistochemistry (4-8 µg/ml), immunocytochemistry (4-8 µg/ml). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps