anti-Lamin B1 Antibody (Clone ZL-5)

Lamin B1

LMNB1 , LMN , LMNB , LMN2 , Lamin-B1 , ADLD , MGC111419

Lamins are classified into A- and B- type isoforms. Specifically, four distinct lamin isoforms have been identified: A, B1, B2, and C. Lamin A and C are alternatively spliced products of a single gene, whereas lamin B1 and B2 are encoded by distinct genes. During mitosis, lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is sμggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions (MARs). Mars mediate the interaction of chromatin with the nuclear matrix. Importantly, some of the same processes that involve mitotic chromosome condensation and nuclear envelop breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.

Mouse

Monoclonal

Mouse IgG1-Kappa

ZL-5

Human, mouse, rat

Recombinant human Lamin B

0.2 mg/ml

Liquid

100 µg (200 µg/ml) protein G purified antibody in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol.

Affinity purified

Western blot, Immunoprecipitation, Immunofluorescence, ELISA

Western blotting (0.5-2 µg/ml), immunoprecipitation (5 µg/ml), immuno-fluorescence (10-15 µg/ml), and ELISA (0.2-1 µg/ml). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps