anti-Calcineurin A Antibody
Catalog number
3075-100
Name
anti-Calcineurin A Antibody
Size
Calcineurin A Antibody
Price
338.00 EUR
Supplier
Details
Target of this antibody:
Calcineurin A
Alternative names of antibody target:
PPP3CA, CCN1, CNA1, CALN, CALNA, CALNA1, PPP2B
Background information:
Calcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drμgs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat, hamster, rabbit, bovine
Immunogene sequence:
Synthetic peptide corresponding to residues surrounding amino acids 267 of human Calcineurin A
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.2 mg/ml) affinity purified, rabbit anti-Calcineurin A polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Recommended usage:
Western Blotting (1-4 µg/ml) and for immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A..
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps
