anti-Hsp27 Antibody

Catalog number
3093-100
Name
anti-Hsp27 Antibody
Size
Hsp27 Antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
Hsp27
Alternative names of antibody target:
HSPB1, CMT2F, DKFZp586P1322, HS.76067, HSP27, HSP28, Hsp25
Background information:
Hsp27 exhibits a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines, glycines or aspartates, have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Rat
Immunogene sequence:
Synthetic peptide surrounding amino acid 193 of rat Hsp2
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 27 kDa rat Hsp27. Rat kidney tissue lysate can be used as a positive control.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps