anti-AMPK⍺1 Antibody

AMPK⍺1

5'-AMP-activated protein kinase, catalytic alpha-1 chain, AMPK alpha-1 chain

AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse

Synthetic peptide selected from the C-terminal region of human AMPK alpha 1

0.25 mg/ml

Liquid

100 µg (0.25 mg/ml) purified rabbit Ig polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Affinity purified

ELISA, Western blot

The antibody can be used for ELISA (0.25 µg/ml) and Western blotting (0.5 – 2.5 µg/ml).

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps