anti-Caspase-7 (Active) Antibody

Caspase-7 (Active)

CASP7,CASP-7,ICE-LAP3,CMH-1,MCH3, EC 3.4.22.60

Caspase-7 has been identified as a major contributor to the execution of apoptosis. Caspase-7 is cleaved by many enzymes, including caspase-3, -6, -8, -9 and granzyme B. Active caspase-7 consists of a heterotetramer of two large and two small subunits. As an effector caspase, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase (PARP). The active caspase-7 antibody provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 195 of human caspase-7

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified rabbit anti-Active caspase-7 antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal

Affinity purified

Western blot,Immunoprecipitation, Immunohistochemistry

Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody detects only the large subunit of cleaved caspase-7. The antibody does not recognize full length of caspase-7 or other caspases.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps