EZBlock™ T20 (PBS) Blocking Buffer

Contents: T20 (PBS) [Phosphate Buffered Saline] Blocking Buffer containing proprietary protein and 0.05% Tween-20

BioVision’s EZBlock™ T20 (PBS) Blocking Buffer is based on a proprietary protein, which efficiently blocks residual binding sites in ELISA, immunohistochemistry and Western blotting applications. This blocking buffer contains Tween 20, which improves blocking performance in many detection systems. In addition, this blocking buffer is albumin or endogenous biotin free, which makes it compatible with avidin/streptavidin systems and delivers high signal-to-noise ratio in most systems. Notes: a. In certain applications, optimized buffer concentration may be beneficial. For example, if the blocking buffer is used as a diluent for antibodies to improve signal-to-noise ratios, the buffers may be used as supplied or diluted up to 10-fold. b. EZBlock™ T20 (PBS) Blocking Buffer may be used as a protein stabilizer for drying antigen or antibody coated microplates. c. Selecting the proper blocking buffer can increase sensitivity and prevent nonspecific signal caused by cross-reactivity between the antibody and the blocking reagent. Testing is essential to determine the appropriate blocking buffer for each Western blot system because no blocking reagent is optimal for all systems.

Blocking Buffer

+4°C

gel pack

12 months

You can block the antibody by the specific target amino acid sequence of peptide.This inhibitor of proteases will block aspartic protease, cysteine protease, serine proteases, metalloproteases and aminopeptidases. Lyophilized, EDTA free.

Buffering solutions are useful to keep the pH range sable when using this reagent of Biovision.

Phosphate-buffered saline (abbreviated PBS) is a buffer solution and Phosphate Buffered Saline (PBS buffer), pH 7.4, stabilized is a water-based salt solution containing sodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate.