anti-IKKalpha/IKK-1 Antibody

IKKa/IKK-1

IKK-alpha, IKKA, IKK-A, IKBKA, NFKBIKA, TCF16, CHUK, IKK1

IKKα (IkB kinase-α or IKK-1) is part of a large protein complex responsible for the inducible phosphorylation of IkB proteins. Human IKK-α is an 85 kDa peptide that has been shown to activate NFkB by phosphorylation of IkB proteins. IKKα interacts with its upstream kinase, NIK, and its downstream substrate, the IkB proteins. Mutations of IKKα in its kinase domain lead to a dominant-negative phenotype that suppresses TNF-α and IL-1β induced NFkB activation.

Rabbit

Polyclonal

Rabbit IgG

Human

E. coli expressed recombinant human IKKa

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) Protein A purified rabbit anti-IKKα polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunoprecipitation

Western blot (at 0.5-1 µg/ml) and immunoprecipitation (4-8 µg/ml). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps