anti-Cathepsin B Antibody

Cathepsin B

CTSB, CPSB, APPS, EC 3.4.22.1

Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy sμggesting that this enzyme may be a useful prognostic marker for several types of human cancers.

Rabbit

Polyclonal

Rabbit IgG

Human

Synthetic peptide surrounding amino acid 322 of human cathepsins B

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified rabbit anti-cathepsin B polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot, Immunohistochemistry, Flow cytometry

Western blotting (0.5-4 µg/ml) amd in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of cathepsin B. It reacts with human and mouse and in a lesser extent with rat samples.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

anticorps