anti-Bcl-2 Antibody (Clone Bcl-2/100)

Bcl-2

BCL2, BCL 2

Bcl-2 (B-cell leukemia/lymphoma 2) gene was discovered by walking the chromosomal breakpoints t(14;18). This gene consists of two exons and several transcripts that encode two different protein products, Bcl-2α and Bcl-2β. Both proteins are approximately 26-30 kDa and differ at their C-terminal region. Bcl-2 suppresses apoptosis (programmed cell death) and this property appears to be modulated by homodimerization or association with Bax and Bad, two cell death promoters

Mouse

Monoclonal

Mouse IgG1

Bcl-2/100

Human

Synthetic peptide corresponding to resides surrounding a.a. 44 of Human Bcl-2

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95%. The antibody was formulated in PBS containing 1 mg/ml BSA and 1.5 mM sodium azide and 50% glycerol

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blot (1-4 µg/ml), Immunoprecipitation, Immunohistochemistry, and Flow cytometry. However the optimal conditions should be determined individually. Recognize the 26 kDa human Bcl-2

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps