anti-Bnip3L/Nix Antibody

Bnip3L/Nix

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; NIP3-like protein X

Members of Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3α, and Nix (for Nip3-like protein X). Bnip3L/Bnip3α/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes supression by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The message RNA of Bnip3L and Bnip3 form a new subfamily of the proapoptotic mitochondrial proteins.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse

Synthetic peptide surrounding amino acid 80 of human Bnip3L.

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit anti-Bnip3L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunohistochemistry

The antibody can be used for Western blot analysis (0.5-2 µg/ml) and immunohistochemistry (2-10 µg/ml). The optimal conditions should be determined individually. Other applications have not been tested.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps