anti-Gai2 Antibody

Gai2

guanine nucleotide binding protein G, GNAI2B, guanine nucleotide binding protein

Gαi2 is a 40-41 kDa alpha-subunit of heterotrimeric protein that shares sequence homology with Gαi3 and Gαi1. All these G proteins can be ADP-ribosylated at the carboxy terminal Cys of the CAAX sequence, interfering with receptor coupling. Gαi2 is expressed widely in tissues and cell lines. Activation of PLC-β by Gαi-coupled receptors is the result of release of Gβg subunits from Gαi. Gαi2 regulates potassium channel activation. Mutation at Arg179 has been identified in tumors. This and the Q205L mutant inhibit adenylyl cyclase.

Rabbit

Polyclonal

Rabbit IgG

Human

Synthetic peptide surrounding amino acid 229 of human Gai2

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity-purified rabbit anti-Gαi2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot, Immunoprecipitation

Western blot (at 0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps