anti-Ga15/16 Antibody

Ga 15/16

G alpha binding protein

Gα16 is the only heterotrimeric G protein with a restricted expression pattern in hematopoietic cells. Differentiation of promyelocytic cells leads to decreased expression of Gα16. The G protein is known to couple a large number of G protein-coupled receptors to PLC-β and can lead to production of the secondary messengers diacylglycerol and inositol phosphates. Targeted deletion of Gα15, a mouse orthologue of human Gα16, causes reduced C5α receptor signaling. Gα16 serves as a marker, in addition to CD34, for hematopoietic progenitor cells.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 356of human G 15

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit anti-Gα15 polyclonal antibody in PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml) and in Immunohistochemistry (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Gα15 in samples from human, mouse, rat, and rabbit origins. It also detects Gα16 from samples of human origin. Reactivity to other species has not been determined.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps