EZDetect™ Polyubiquitin Probe (Biotin conjugated)

250 µl (100 µg) of Biotin-UIM-UBA protein (0.4 mg/ml) in PBS with 0.1% Azide. Applications: • To detect the poly-ubiquitinated proteins in cell lysate/tissue homogenate using Dot blot. • To detect the poly-ubiquitinated proteins in cell lysate/tissue homogenate using Western blot. • In Immunocytochemistry / Immunohistochemistry studies to label / visualize intracellular poly-ubiquitinated proteins.

BioVision’s EZDetect™ Polyubiquitin Probe is ideal for detecting poly-ubiquitinated proteins in biological samples. Ubiquitin is a highly conserved 76-amino acid protein. It can be conjugated via its C-terminus to the amine groups of lysine residues on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. Different types of polyubiquitin chains can form, depending on the internal lysine residue used for this conjugation. These polyubiquitin chains further can attach to proteins post-translationally and aid in numerous downstream activities like proteasome-mediated proteolysis, autophagy, DNA damage tolerance, inflammation, apoptosis, signal transduction etc. Several classes of Ubiquitin interacting proteins help in mediating these downstream effects. Ubiquitin Interacting Motifs (UIM) and Ubiquitin Associated Domains (UBA) are two large classes of such protein domains which strongly interact with polyubiquitin chains. We have developed an UIM-UBA chimeric protein from the UIM and UBA domains. Both these proteins are well-characterized for their high-affinity interaction with different types of polyubiquitin chains. The EZDetect™ Polyubiquitin Probe is synthesized through biotinylation of UIM-UBA with Sulfo-NHS-LC-Biotin (Cat # 2326). This biotin-UIM-UBA can be used to detect poly-ubiquitinated proteins by decreasing the interference from free monomeric ubiquitin which is highly abundant in biological samples.

EZDetect™ Polyubiquitin Probe

+4°C

gel pack

12 months

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Biotinylated

any of various testing devices or substances as (1)   a pointed metal tip for making electrical contact with a circuit element being checked (2)   a usually small object that is inserted into something so as to test conditions at a given point (3)   a device used to penetrate or send back information especially from outer space or a celestial body (4)   a device (as an ultrasound generator) or a substance (as radioactively labeled DNA) used to obtain specific information for diagnostic or experimental purposes