anti-Phospho-Bad Antibody

Bad

BAD,phospho BBC2, phosphoBBC6, BAD (Phospho-Ser112),BCL2L8

Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic phospho-peptide corresponding to residues surrounding Ser112 of mouse Bad

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) peptide affinity purified rabbit anti-Phospho-Bad (Ser112) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blot analysis (1-2 µg/ml) and immunoprecipitation (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps