anti-IkBalpha Antibody (Clone 6A055)

Catalog number
3315-100
Name
anti-IkBalpha Antibody (Clone 6A055)
Size
IkBalpha Antibody (Clone 6A055)
Price
371.00 EUR
Supplier

Details

Target of this antibody:
IkBalpha
Alternative names of antibody target:
IkappaBalpha , MAD3, MAD-3, IKBA, NFKBI, IKB-alpha, I-kappa-B-alpha
Background information:
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/g. External stimuli such TNF-α or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBα.
Antibody host:
Mouse
Clonality of antibody:
Monoclonal
Isotype:
Mouse IgG1
Clone:
6A055
This antibody reacts with:
Human
Immunogene sequence:
Recombinant human IkBa
Concentration of antibody:
0.25 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.25 mg/ml) immunoaffinity purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blotting (0.5-4 µg/ml) and immunoprecipitation (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects both phosphorylated and non-phosphorylated forms of IkBα.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps