anti-PCNA Antibody

PCNA

PCNA, proliferating cell nuclear antigen

PCNA (Proliferating Cell Nuclear Antigen) is a 36 kDa protein also known as cyclin. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. There are two forms of PCNA protein present in cells, a soluble form sensitive to organic fixation and not involved in replication, and the other form is insoluble and is associated with ongoing DNA synthesis.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide corresponding to the C-terminus of human PCNA

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified rabbit anti-PCNA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blotting (0.5-4 µg/ml). Per researcher’s feedback, it can also be used in immunoprecipitation and Immunohistochemistry (frozen & paraffin). However, the optimal conditions should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps