anti-Annexin V Antibody

Annexin V

ANXA5, ANX5, ENX2, PP4

The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Calcium dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in culture.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Recombinant human Annexin V

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit anti-Annexin V polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot,Immunoprecipitation, Immunohistochemistry

Western blotting (0.5-4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. The antibody detects annexin V from samples of human, mouse, and rat origin. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used as positive controls.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps