anti-Phospho-ATF-2 Antibody

Phospho-ATF-2 (Thr71)

ATF2, HB16, CREB2, CREBP1, CRE-BP1, CRE-BP1, TREB7, MGC111558, Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; cAMP response element-binding protein CRE-BP1; HB16; Cyclic AMP-responsive element-binding protein 2; cAMP-responsive element-binding protein 2; CREB-2.

The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) purified rabbit anti-Phospho-ATF-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blotting (1-4 µg/ml), immunoprecipitation (10 µg/ml), and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated ATF-2. It recognizes both Thr69/Thr71 dually phosphoralated ATF-2 as well as ATF-2 singly phosphorylated ATF-2 in samples of human, mouse, and rat origins.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps