anti-D4-GDI Antibody

D4-GDI

ARHGDIB , 602843 , P52566 , D4 , GDIA2 , GDID4 , LYGDI , Ly-GDI , RAP1GN1

D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeleytal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block throμgh its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28kD mature form of D4-GDI to give a 23kDa and 5kDa size fragment. The 23kDa fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Rabbit

Polyclonal

Rabbit IgG

Human

Synthetic peptide near the N-terminus of human D4-GDI

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 28 kDa D4-GDI in human samples. Jurkat cell lysate can be used as a positive control.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps