anti-Hsp90 Antibody

Hsp90

HSP90AA1 , HSP90A , HSPCAL1 , HSPCAL4 , HSPN , HSPCA , HSP90N , LAP2 , HSPC1 , FLJ31884

Heat shock proteins (HSPs) are ubiquitously expressed in all organisms. A major function of HSP90 and other HSPs is to act as molecular chaperones. HSP90 forms a complex with glucocorticoid receptor (GR), rendering the non ligand-bound receptor transcriptionally inactive. HSP 90 binds the GR as a heterocomplex composed of either HSP56 or cyclophilin-40, forming an aporeceptor complex. HSP90 also exists as a dimer with other proteins such as p60/sti1 and p23, forming an aporeceptor complex with estrogen and androgen receptors.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 714 of human Hsp90

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blotting, Immunoprecipitation, Immunohistochemistry

Western blotting (0.5-4 µg/ml), in immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (frozen sections, 10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes Hsp90α and Hsp90β of human, mouse, and rat origins.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps