anti-Caspase-10 Antibody

Caspase-10

CASP10 , ALPS2 , MCH4 , FLICE2 ,CASP-10 , ICE-like apoptotic protease 4

Caspase family of cysteine proteases play a key role in apoptosis. Caspase-10 is expressed in many tissues and cell lines, and has been identified as caspase-10/a (short form) and caspase-10/b (long form), respectively. Similar as other caspases, caspase-10 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Activated caspase-10 activates caspase-3,-4,-6,-7,-8, and –9, which are then involved in proteolytic cleavage of many cellular proteins. Caspase-10 has been shown involving in the Fas and TNFR1 induced apoptosis.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 404 of human caspase-10

0.2 mg/ml

Liquid

200 µg (200 µg/ml) affinity purified rabbit anti-caspase-10 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects the proform (~55 kDa), intermediates (30 kDa and 34 kDa), and cleaved form (~20 kDa) of caspase-10 on SDS-PAGE immunoblots.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps