anti-ILK1 Antibody

ILK1

p59ILK , ILK-1 , P59 , DKFZp686F1765

ILKs (Integrin-linked kinases) in combination with integrins and growth factors regulate cell survival, cell cycle, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extra-cellular matrix (ECM) and growth factor receptors to the actin cytoskeleton throμgh interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. ILK phosphorylates several cellular targets including Akt, GSK-3, myosin light chain 2, as well as affixin. These phosphorylation events are key regulatory steps in modulating the activities of the targets.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide corresponding to the C-terminus of human ILK-1

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot, Immunohistochemistry

Western blotting (0.5-4 µg/ml), Immunohistochemistry (10-20 µg/ml, frozen & paraffin). However, the optimal concentrations should be determined individually. The antibody recognizes 59 kDa of ILK1 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps