anti-MSH2 Antibody

MSH2

HNPCC , COCA1 , Flow cytometryC1 , HNPCC1 , BAT-26 , Familial Nonpolyposis Colon Cancer Type 1 , Colorectal Cancer Type 1

DNA mismatch repair genes have been found to be associated with hereditary nonpolyposis colorectal cancer (HNPCC). Inherited mutations in the MSH2 were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability sμggests that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide mapping to a region between 1-50 of MSH2

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blotting (1-4 µg/ml), immunoprecipitation (15-25 µg/ml), and Immunohistochemistry (15-25 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the MSH2 of human, mouse, and rat origins. Reactivity to other species has not been tested.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps