anti-MLK3 Antibody

MLK3

MAP3K11 , MLK3 , SPRK , MLK-3 , PTK1 , MGC17114

MLK3 (Mixed lineage kinase 3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, althoμgh it is controversial whether MLK3 is involved in p38 stress pathway activation. MLK3 also functions as an IkB kinase and mediates the activation of the transcriptional factor NF-kB stimulated by CD3/CD28, sμggesting a role for MLK3 in immune and inflammatory responses.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide mapping to the C-terminus of human MLK3.

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 92 kDa MLK3 of human, mouse, and rat origins. It does not cross-react with MLK1, MLK2 and other mixed lineage kinases.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps