anti-Tau-13 Antibody (Clone B11E8)

Tau-13

FLJ31424 , FTDP 17 , FTDP17 , MSTD , MAPT

Tau, a microtubule-binding protein which serves to stabilize microtubules in growing axons, is found to be hyperphosphorylated in paired helical filaments (PHF), the major fibrous component of neurofibrillary lesions associated with Alzheimer’s disease. Hyperphosphorylation of Tau is thoμght to be the critical event leading to the assembly of PHF. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK3. This presents the possibility that miscues in GSK3 signaling contribute to the onset of Alzheimer’s disease.

Mouse

Monoclonal

Mouse IgG1

B11E8

Human

Synthetic peptide corresponding to the N-terminus of the longest isoform of human Tau

N/A

Liquid

100 µl mouse ascites diluted in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blotting (1:1000), immunoprecipitation (1:500); and Immunohistochemistry (1:100). However, the optimal concentrations should be determined individually. The antibody recognizes 45-60 kDa human Tau-13. Reactivity to other species has not been tested.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps