anti-GSK-3a Antibody

GSK-3a

DKFZp686D0638, GSK-3alpha, Glycogen synthase kinase-3, GSK-3 alpha

Glycogen synthase kinase 3 (GSK-3) is a serine/threonine protein kinase that has been implicated in the regulation of cell fate and in the Wnt signaling pathway. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has also been implicated in alzheimer’s disease. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide from human GSK-3alpha

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity-purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Affinity purified

Western blotting, Immunoprecipitation, Immunohistochemistry

Western blot analysis (1-2 µg/ml) and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects GSK-3 from human, mouse, & rat origin; non cross-reactive with GSK-3β.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps