anti-Phospho-Lck Antibody

Phospho-Lck

LCK , p56-LCK , p56lck , YT16 , pp58lck , LSK

Lck is a membrane associated tyrosine protein kinases. The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas. In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Resting T cells contain high levels of the Lck protein and mRNA both of which decline upon activation of the T cells. These and other observations sμggest that alterations in Lck expression may contribute to the pathogenesis of some types of neoplastic disease.

Rabbit

Polyclonal

Rabbit IgG

Human

Synthetic peptide corresponding to amino acids around Tyr505 of human Lck

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

Affinity purified

Immunoprecipitation, Immunohistochemistry

Western blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (20-25 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa phosphorylated Lck (Tyr505) of human, mouse and rat origins. Reactivity to other species has not been tested.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps