anti-MMP-9 Antibody

MMP-9

GELB , EC 3.4.24.35 , CLG4B , Matrix metalloproteinase

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92-kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 110 of human MMP-9

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the full length (proenzyme, 92 kDa) and cleaved (active enzyme, 84 kDa) MMP-9 in samples from human, mouse, and rat origins.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps