anti-NSF Antibody

NSF

SKD2 , N-ethylmaleimide-sensitive Fusion Protein , Anti-NSF, NSF

Syntaxins were originally thoμght to be docking proteins, but have more recently been categorized as anchoring proteins that anchor themselves to the cytoplasmic surfaces of cellular membranes. Syntaxins have been shown to bind to various proteins involved in exocytosis, including VAMPs (vesicle-associated membrane proteins), NSF (N-ethylmaleimidesensitive factor), SNAP 25 (synaptosomal-associated protein of 25kDa), SNAPs (soluble NSF attachment proteins) and synaptotagmin. SNAPs mediate the membrane binding of NSF, which is essential for membrane fusion reactions. An additional protein designated synaptophysin may regulate exocytosis by competing with SNAP 25 and syntaxins for VAMP binding.

Rabbit

Polyclonal

Rabbit IgG

Mouse, rat

Synthetic peptide corresponding to amino acids around 731 of rat NSF

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.

Affinity purified

Western blot, Immunoprecipitation, Immunohistochemistry

Western blotting (1-2 µg/ml), immunoprecipitation (5-15 mg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 85 kDa NSF of mouse, and rat origins. Reactivity to other species has not been tested

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps