anti-TLR2 Antibody (Clone BV31-9)

TLR2

Toll-like receptor 2 , TLR2 , Anti-TLR2 , CD282 , TIL4

The Toll-like receptor (TLR) family in mammalian comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Up-to-date, ten TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells. CD3+ T-cells and CD56+ NK cells did not express TLR2. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulate TLR2, whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Mouse

Monoclonal

Mouse IgG1

BV31-9

Human

Recombinant human TLR2

0.5 mg/ml

Liquid

100 µl protein G purified mouse anti-human TLR2 monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Western blot

Western blotting (1:200-1000 dilution). However, the optimal conditions should be determined individually. Ramos cell lysate can be used as a positive control and a ~86 kDa band is detected.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps