anti-Oct-2 Antibody

41914

OCT2 , OTF2 , Oct-2 , POU2F2

Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide mapping to the c-terminus of human Oct-2

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Affinity purified

Western blot,

Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~52 kDa Oct-2 in samples from human, mouse, and rat origins. Reactivity to other species has not been tested.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps