anti-Oct-4 Antibody

Catalog number
3576-100
Name
anti-Oct-4 Antibody
Size
Oct-4 Antibody
Price
403.00 EUR
Supplier

Details

Target of this antibody:
41916
Alternative names of antibody target:
MGC22487, OCT3, OTF3, OTF4, Oct4 , POU5F1 , Oct-3
Background information:
Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide mapping to the N-terminus of mouse Oct-4
Concentration of antibody:
0.5 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100 µg (0.5 mg/ml) affinity purified rabbit anti-Oct-3/4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.03% ProClin®
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunohistochemistry
Recommended usage:
Western blotting (0.5-4 µg/ml), immunoprecipitation (20 µg/ml) and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with 45 kDa Oct-4 (also designated Oct-3A) and in a less extent 33 kDa Oct-3B from human, mouse, and rat samples. Rat kidney tissue lysate can be used as a positive control.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps