ToxOut™ Rapid Endotoxin Removal Kit

• High Binding Capacity: up to 9 x 108 EU/ml resin • High Sample Recovery: ~90% recovery with protein solution samples • Column Content: 5 x 100 µl ToxOutTM Rapid Endotoxin Removal Agarose (Cat. No. 7941-5) • Resin Content: affinity matrix of polymyxin B, supplied as 50% slurry in 20% ethanol • Each column can be reused up to 5 times • Rapid removal: only require 5 minutes for each removal cycle

Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules (2 – 20 fg/cell). During experimental procedures, large amount of endotoxins are shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products. BioVision‘s ToxOutTM Rapid Endotoxin Removal Kit can quickly and effectively eliminate endotoxins to < 0.05 EU/ml in solutions containing proteins or pharmacologically important components via the immobilized polymyxin B, which is known for capturing endotoxin and preventing toxic effects.

ToxOut / endotoxin-free

4°C Do not freeze

gel pack

12 months