ToxOut™ High Capacity Endotoxin Removal Kit

• High Binding Capacity: up to 1.5 x 10^9 EU/ml resin • High Sample Recovery: ~97% recovery with protein solution samples • Columns Content: 5 x 100 µl ToxOutTM High Capacity Endotoxin Removal Agarose (Cat. No. 7942-5) • Resin Content: affinity matrix of poly-ε-lysine, supplied as 50% slurry in 20% ethanol • Each column can be reused up to 5 times (The buffers provided in the kit is sufficient for single use.) • Final products are more suitable for potential therapeutic applications as ligand and buffers are nontoxic.

Endotoxin is the lipopolysaccharide (LPS) complex located in the outer membrane of gram-negative bacteria. A single E.coli bacterium contains ~2 million LPS molecules. During experimental procedures, large amount of endotoxins are shed and can easily contaminate labware, buffers and downstream products. In vitro, endotoxin causes a variety of problems in cell-based research. In vivo, endotoxin may cause various side effects, including inflammatory response, organ failure or septic shock in host organisms. Therefore, it is critical to remove endotoxin from samples and products. BioVision‘s ToxOutTM High Capacity Endotoxin Removal Kit can effectively decrease high concentration to levels lower than < 0.05 EU/ml without using toxic buffers while maintaining the protein recovery rate at >97%. BioVision‘s ToxOutTM High Capacity Endotoxin Removal Agarose in this kit is using poly-ε-lysine as affinity ligand. It is a safe, nontoxic food preservative known for capturing and removing endotoxin from samples.

ToxOut / endotoxin-free

4°C Do not freeze

gel pack

12 months