anti-HDAC7 Antibody

HDAC7

HDAC7A, histone deacetylase 7A

Human HDAC7 is composed of 912 amino acid residues. Althoμgh HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, sμggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 921 of murine HDAC7

0.2 mg/ml

Liquid

100 µg (0.2 mg/ml) affinity purified rabbit anti-HDAC7 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blotting, Immunoprecipitation, Immunohistochemistry

Western blotting (0.5-4 µg/ml), immunoprecipitation (20-40 µg/ml) and Immunohistochemistry (20-40 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~105 kDa HDAC7. Mouse small intestine tissue lysate can be use as a positive control. The antibody does not recognize other members of the HDAC family.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps