EZCut™ SUMO Protease 1 Agarose Beads

EZCut™ SUMO Protease 1 Agarose Beads are designed for efficient cleavage of recombinant fusion proteins, containing a SUMO tag, circumventing the need for additional chromatographic step(s) to remove the protease after cleavage reaction is completed.

SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s EZCut™ SUMO Protease 1 Agarose is prepared by covalent coupling of SUMO Protease 1 to activated 4% cross-linked agarose beads. EZCut™ SUMO Protease 1 Agarose Beads are designed for efficient cleavage of recombinant fusion proteins, containing a SUMO tag, circumventing the need for additional chromatographic step(s) to remove the protease after cleavage reaction is completed. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. These beads can be regenerated for repeated use and the reusability depending on the usage and handling of the product.

Protein purification

-20°C

gel pack

6 months

The purest agarose was used in the production of EZCut™ SUMO Protease 1 Agarose by Biovision.

Agarose