EZCut™ HRV14 3C (Precission) Protease Agarose Beads

EZCut™ HRV14 3C (Precission) Protease Agarose Beads are designed for efficient cleavage of recombinant fusion proteins, containing the Precission protease cleavage site, circumventing the need for chromatographic techniques to remove the protease after cleavage reaction is completed.

Active HRV14 3C Protease (Precission Protease) domain from human rhinovirus type 14 has high selectivity and site-specificity against Precission cleavage site (Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro), thereby allowing for the production of recombinant proteins with minimal number of extra amino acids. BioVision’s EZCut™ HRV14 3C (Precission) Protease Agarose is prepared by covalent coupling active HRV14 3C Protease to activated 4% cross-linked agarose beads. EZCut™ HRV14 3C (Precission) Protease Agarose Beads are designed for efficient cleavage of recombinant fusion proteins, containing the Precission protease cleavage site, circumventing the need for chromatographic techniques to remove the protease after cleavage reaction is completed. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature. These beads can be regenerated for repeat use and the reusability depends on the usage and handling of the product.

Protein purification

-20°C

gel pack

6 months

The purest agarose was used in the production of EZCut™ HRV14 3C (Precission) Protease Agarose by Biovision.

Agarose