anti-JIP-1 Antibody

Catalog number
3664-100
Name
anti-JIP-1 Antibody
Size
JIP-1 Antibody
Price
316.00 EUR
Supplier

Details

Target of this antibody:
JIP1
Alternative names of antibody target:
JIP 1 , JNK , IB1 , IB 1 , MAPK8IP 1 , MAPK8IP1 , Mitogen activated protein kinase 8 ,
Background information:
JIP1 (JNK-interacting protein-1) is a cytoplasmic protein that possesses an N-terminal JNK-binding domain and a C-terminal SRC homology 3 (SH3) domain. JIP1 selectively bind to JNK, but not to the ERK or p38 MAP kinases. In addition to JNK, JIP1 binds to specific proteins of JNK pathways, including MKK7, MLK3, and DLK, all at separate binding sites. JIP1 has been identified in brain and kidney tissues, whereas JIP2a, JIP2b, and JIP3 are specifically expressed in the brain. Overexpression of JIP1 causes cytoplamic retention of JNK, thereby preventing its nuclear accumulation and the subsequent activation of the JNK pathways, indicating that JIP1 acts as a cytoplasmic anchor for JNK.
Antibody host:
Rabbit
Clonality of antibody:
Polyclonal
Isotype:
Rabbit IgG
This antibody reacts with:
Human, mouse, rat
Immunogene sequence:
Synthetic peptide from mouse JIP1
Concentration of antibody:
0.2 mg/ml
Form of antibody:
Liquid
Formulation of this antibody:
100µg (0.2mg/ml) protein A purified rabbit anti-JIP1 polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5% BSA, 30% glycerol, and 0.01% thimerosal.
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot
Recommended usage:
Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 78 kDa JIP1 in samples from mouse, rat and human origins. In human sample, a ~110 kDa isoform can also be observed. Reactivity to other species has not been tested.
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions:
gel pack
How to handle this antibody:
The antibody solution should be gently mixed before use.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation
anticorps