anti-Phospho-VASP Antibody

Phospho-VASP

Vasodilator-stimulated phosphoprotein

Vasodilator-stimulated phosphoprotein (VASP) belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways. VASP functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance. Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser157 is the major PKA phosphorylation site. Evidence sμggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic phospho peptide surrounding amino acid Ser157 of human VASP

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in 1X phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml) and Immunohistochemistry. However, the optimal concentrations should be determined individually. The antibody recognizes 51 kDa phosphorylated VASP from samples of human, mouse, and rat origins. Reactivity to other species has not been tested.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps