anti-Prohibitin Antibody

Prohibitin

PHB, phb

Prohibitin is an evolutionarily conserved protein that has anti-proliferative activity. Prohibitin is a 30 kDa, post-synthetically modified protein found in the inner membrane of mitochondria and on the plasma membrane of B cells. It mediates B cell mutation. Prohibitin Controls cell cycle in both transcriptional and post-translational mechanisms. Prohibitin is a potential tumor suppressor. Prohibitin gene mutations are correlated with the breast cancer development and/or progression in over 80%0f the cell lines analyzed.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surrounding amino acid 256 of human Prohibitin

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, Immunofluorescence

Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunofluorescence (5-10 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 33 kDa protein, corresponding to the apparent molecular mass of Prohibitin on SDS-PAGE immunoblots, in samples from human, mouse and rat origins.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps