anti-Tim-3 Antibody

Tim

tim-3, tim3, tim 3, TIMD3, TIMD-3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell membrane protein 3

As a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. It is known to synergize with Toll-like receptors and is thought to regulate Th1 and Th17 cytokine secretion.

Rabbit

Polyclonal

Rabbit IgG

Human

Synthetic peptide surrounding amino acid residue 285 of human Tim-3

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Affinity purified

Western blot and Immunohistochemistry (5 µg/ml)

Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 33 kDa human Tim-3 on SDS-PAGE immunoblots. Other applications have not been determined.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps