anti-ATGLAntibody

ATGL

Adipose Triglyceride Lipase, Patatin-like phospholipase domain-containing protein 2, Desnutrin, Transport-secretion protein 2, TTS2

Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues. Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific. ATGL was independently identified as desnutrin and the TG-hydrolace inducible phospholipase-A2-z.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide corresponding to residues surrounding amino acids 385 of human ATGL

0.5 mg/ml

Liquid

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Affinity purified

Western blot, Immunoprecipitation, and Immunohistochemistry

Western blotting (0.5-4 µg/ml), Immunohistochemistry (5 µg/ml), and immunoprecipitation. recognizes 54 kDa ATGL in samples from human, mouse and rat origins. However, the optimal concentrations should be determined individually.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.