anti-APG7/ATG7 Antibody

ATG7/APG7

APG7L , ATG7 , DKFZp434N0735 , GSA7

Autophagy, the process of bulk degradation of cellular proteins throμgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) throμgh phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4). In mammalian cells, APG7 is essential for autophagy conjμgation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been sμggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse

Synthetic peptide near the N-terminus of rat APG7

0.5 mg/ml

Liquid

0.5 mg/ml affinity purified rabbit anti-APG7/ATG7 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps