anti-APG10/ATG10 Antibody

APG10/ATG10

APG10 , APG10L, ATG10 , DKFZP586I0418 , FLJ13954 , pp12616

Autophagy, the process of bulk degradation of cellular proteins throμgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) throμgh phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is APG10 (Also called ATG10, APG10L), an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjμgation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide, near the carboxyl terminus of human APG10

0.5 mg/ml

Liquid

0.5 mg/ml affinity purified rabbit anti-APG10 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.

Affinity purified

Western blot

Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps