anti-AMPK⍺1 Antibody

AMPK⍺1

5'-AMP-activated protein kinase, AMPK catalytic alpha-1 chain, AMPK alpha-1 chain

AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.

Rabbit

Polyclonal

Rabbit IgG

Human, mouse, rat

Synthetic peptide surroundign amino acid 486 of human AMPK alpha 1

0.5 mg/ml

Liquid

200 µg (0.5 mg/ml) affinity purified rabbit anti-AMPKa1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal

Affinity purified

Western blot

The antibody can be used for Western blotting (1–4 µg/ml).

Shelf time: 12 months in temp.: -20 deg.C

gel pack

The antibody solution should be gently mixed before use.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

anticorps